Victoria Momenabadi; Mohammad Hossein Kaveh; Nouzar Nakhaee; Kambiz Karimzadeh Shirazi; Maryam Dastoorpoor; Behnaz Sedighi
Volume 21, Issue 12 , 2019, Pages 1-15
Abstract
Background: Multiple sclerosis (MS) is a progressive central nervous system disease that has an enormous effect on a patient’s quality of life due to physical complications and psychological problems.Objectives: The purpose of this study is to investigate the effect of self-care education program ...
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Background: Multiple sclerosis (MS) is a progressive central nervous system disease that has an enormous effect on a patient’s quality of life due to physical complications and psychological problems.Objectives: The purpose of this study is to investigate the effect of self-care education program based on Health-Promoting Self-Care Behaviors System Model on quality of life, resilience, and sense of coherence in patients with MS.Methods: In this randomized controlled clinical trial, 80 patients with MS referred to hospitals for special diseases in Kerman were enrolled using systematic sampling method and then randomly assigned to the intervention and control groups. The main research instruments, including demographic information checklist, Multiple Sclerosis Quality of Life (MSQOL-54), Connor-Davidson scale, Resilience Scale and Sense of Coherence Scale were completed by subjects in both intervention and control groups. The intervention group participated in 18 training sessions that addressed the needs of patients. The two groups completed the questionnaires one week and two months after intervention again. Generalized Estimating Equations (GEE) (GEE) test was used for data analysis.Results: Pre-intervention results revealed that intervention and control groups were not significantly different in the mean scores of most dimensions of quality of life and sense of coherence (P > 0.05). However, intervention and control groups were signifi- cantly different in mean scores of all dimensions of quality of life, resilience and sense of coherence one week and (P < 0.05) and two months (P < 0.05) after the intervention. The results of GEE test also reflected a meaningful difference in the intervention group in terms of increased mean scores in all dimensions of quality of life (P < 0.05), resilience (69.60 ± 6.62) (P < 0.05) and sense of coherence (74.50 ± 3.52) (P < 0.05) one week and two months after the intervention compared to the baseline. However, this improvement was not observed in the controls (P > 0.05).Conclusions: Teaching health-promoting self-care behaviors system model improves the quality of life, resilience, and sense of coherence in patients with MS. Therefore, it is recommended to plan and implement self-care training interventions in accordance with this model for all patients with MS.
Tayebeh Hadigol; Aligholi Sobhani; Masoud Hemadi; Saeid Nekoonam; Alireza Shams; Bahram Eslami Farsani; Maryam Dastoorpoor; Ghasem Saki
Volume 21, Issue 4 , 2019, Pages 1-9
Abstract
Background: Rutin (quercetin-3-rhamnosyl-glucoside), a flavonoid, is derived from plants and has antioxidant properties.Objectives: This study aimed to evaluate the effect of different concentrations of rutin on mouse ovary heterotopic allotransplan- tation.Methods: The present animal experimental study ...
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Background: Rutin (quercetin-3-rhamnosyl-glucoside), a flavonoid, is derived from plants and has antioxidant properties.Objectives: This study aimed to evaluate the effect of different concentrations of rutin on mouse ovary heterotopic allotransplan- tation.Methods: The present animal experimental study was conducted on 40 female adult Balb/c mice weighing 30 ± 5 g at the Jundisha- pur University of Medical Sciences, Ahvaz, Iran, during 2016 - 2018. The mice were divided by permuted block randomization into 8 groups (n = 5): OVX (ovariectomy), as the negative control; normal (positive control); OVX + OVA (ovariectomy and transplantation) (control), treated with 0.5 mL of normal saline; OVX + OVA + 10 mg/kg of rutin; OVX + OVA + 30 mg/kg of rutin; OVX + OVA + 60 mg/kg of rutin; OVX + OVA + 100 mg/kg of rutin; and the autograft. Groups were treated daily. Fourteen days after transplantation, ovarian grafts were collected and processed histologically for follicle number counting. Serum estrogen (E2) and progesterone (P4) levels were evaluated. Furthermore, the expression of Estrogen Receptor alpha (ERα), Estrogen Receptor beta (ERβ), and Progesterone Receptor (PR) in the uterine endometrial tissue was tested using qRT-PCR and western blotting.Results: A decrease in the number of mature follicles and increase in the number of atretic follicles (mean ± SD: OVX + OVA + 30 = 19.00 ± 1.000, OVX + OVA + 60 = 25.00 ± 5.000, and OVX + OVA + 100 = 23.00 ± 2.646) were observed in all groups treated with rutin in comparison with the control group (mean ± SD: 12.33 ± 2.517) (P value < 0.05). The level of E2 and P4 (mean ± SD: OVX + OVA + 100 = 6.133 ± 1.026) increased in comparison with the OVX + OVA group (mean ± SD: 0.4667 ± 0.2517) (P value < 0.05). The protein expression of ERα (mean ± SD: OVX + OVA + 10 = 1.615 ± 0.1701 and OVX + OVA + 30 = 1.744 ± 0.1779) in comparison with the control group (mean ± SD: 0.7089 ± 0.1131), and ERβ (mean ± SD: OVX + OVA + 10 = 0.7747 ± 0.4365, OVX + OVA + 30 = 0.9220 ± 0.1245, OVX + OVA + 60 = 0.7701 ± 0.2150, and OVX + OVA + 100 = 0.6676 ± 0.1547) increased in a dose-dependent manner in all groups treatedwith rutin in comparison with the OVX + OVA group (mean ± SD: 0.1534 ± 0.06109) (P value < 0.05). No significant changes in PR were found in groups treated with rutin in comparison with the control group.Conclusions: The results of the present study indicated that rutin increases E2 and P4 levels in ovarian hetero allograft mice. Rutin also upregulated the expression of ERα and ERβ but had no significant effect on PR.
Mahmoud Hashemitabar; Elham Heidari; Mahmoud Orazizadeh; Susan Sabbagh; Mahsa Afrough; Maryam Dastoorpoor; Ata A. Ghadiri
Volume 20, Issue 12 , 2018, Pages 1-10
Abstract
Background: Asthenozoospermia (astheno) is a common male infertility disorder associated with low sperm motility. The pro- gressive movement of sperm is an important factor in the fertilization rate, and it requires a high level of adenosine triphosphate (ATP). Objectives: This experimental study aimed ...
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Background: Asthenozoospermia (astheno) is a common male infertility disorder associated with low sperm motility. The pro- gressive movement of sperm is an important factor in the fertilization rate, and it requires a high level of adenosine triphosphate (ATP). Objectives: This experimental study aimed to identify the role of cytochrome c oxidase 6B2 (COX6B2) as an important functional subunit of Cytochrome-c Oxidase in sperm motility. Methods: According to the World Health Organization (WHO) criteria, Semen samples were collected from 14 asthenozoospermia and 16 normospermia individuals that were referring to the Infertility Research and Treatment Center of Khuzestan, Iran in October2016- May 2017. The sperm from two groups was isolated via the Percoll density gradient centrifugation to prepare healthy, motile sperm for COX6B2 immunofluorescent staining and real-time polymerase chain reaction (PCR). In addition, apoptosis assessment was carried out simultaneously to compare apoptosis and the COX6B2 expression level. To analyze the data, descriptive statistics including frequency and mean and analytical statistics including Fisher’s exact test and two independent samples were used. Results: COX6B2 was detected in midpiece of the sperm by immunofluorescence assays. In addition, the percentage of COX6B2 positive sperm in the astheno samples was almost half that of the normal group (49.0 ± 15.8 to 28.7 ± 14.1, P = 0.641). Real-time PCR definitely reconfirmed the immunofluorescent staining result. A decrease in apoptosis was shown in as the no samples compared with the normal group (19.1 ± 0.4 to 9 ± 0.2, P = 0.04). Conclusions: The expression of COX6B2 in the sperm midpiece represents the OXPHOS pathway and functionality of mitochondria in sperm. This study introduced COX6B2 staining as a potential functional test for the recognition of competent mitochondria in sperm and it could be assigned as a biomarker in male-factor patients.
Seyed Vahid Ahmadi Tabatabaei; Hassan Eftekhar Ardabili; Ali Akbar Haghdoost; Maryam Dastoorpoor; Nouzar Nakhaee; Mohsen Shams
Volume 19, Issue 10 , October 2017, , Pages 1-8
Abstract
Background: Theory of planned behavior (TPB) has not been adequately addressed in the field of physical activity among Iranian women.Objectives: This study aimed to determine factors affecting physical activity behavior among women based on the theory of planned behavior.Methods: This cross-sectional ...
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Background: Theory of planned behavior (TPB) has not been adequately addressed in the field of physical activity among Iranian women.Objectives: This study aimed to determine factors affecting physical activity behavior among women based on the theory of planned behavior.Methods: This cross-sectional study was conducted on 398 women in Kerman, Iran. The women were selected through the multistage random sampling method. Data were collected using the international physical activity questionnaire (IPAQ) and a researchermade questionnaire based on the constructs of the theory of planned behavior and analyzed using structural equation modeling (SEM).Results: The mean age (SD) of participants was 36.9 (9.9). Perceived behavioral control (β = 0. 16, P = 0. 013) and behavioral intention (β = 0.16, P = 0.005) had a significant positive direct effect on physical activity behavior. Moreover, instrumental attitude (β = 0.04, P = 0.003), subjective norms (β = 0.04, P = 0.003) and perceived behavioral control (β = 0.03, P = 0.002) had a significant positive indirect effect on physical activity behavior. Behavioral intention and perceived behavioral control constructs predicted 6% of variance of physical activity behavior.Conclusions: Given that the constructs of behavioral intention and perceived behavioral control could predict physical activity behavior, health promotion interventions should be designed with an emphasis on these factors in order to promote physical activity in women.
